U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX5818177: GSM3760133: 53A_ILC2; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 27.1M spots, 1.6G bases, 700.6Mb downloads

Submitted by: NCBI (GEO)
Study: Group 3 innate lymphoid cells mediate early protective immunity against Mycobacterium tuberculosis
PRJNA542348 • SRP197580 • All experiments • All runs
show Abstracthide Abstract
We report the phenotype of human lung ILC2 and ILC3 populations from individuals with tuberculosis (TB) and non-TB cancer controls. We find that ILC2s demonstrate moderate transcriptional differences in TB infection, whereas ILC3s demonstrate large differences. Overall design: ILC2s and ILC3s were purified by FACS from lung biopsies from TB infected lung tissue and peripheral healthy lung tissue from individuals with cancer. Low-input RNA-seq was performed on 1-3 replicates (dependent on cell number) on 5 individuals with TB infection and 2 controls.
Sample: 53A_ILC2
SAMN11619013 • SRS4746946 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted from cell lysate (RLT +1% BME) using RNAClean XP SPRI beads. Messenger RNA was primed for reverse transcription and PCR using an oligodT RT primer. Libraries were constructed using a modified version of the Smart-Seq2 protocol described in Trombetta et al., Curr Protoc Mol Biol, (2014). Libraries were sequenced twice on a NextSeq 500 using the 75-cycle High Throughput kit.
Links:
Runs: 1 run, 27.1M spots, 1.6G bases, 700.6Mb
Run# of Spots# of BasesSizePublished
SRR904108127,069,6481.6G700.6Mb2019-05-13

ID:
7819218

Supplemental Content

Related information

Recent activity

Clear Turn Off Turn On

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...